Composite
A1-R1-A3 p

Part:BBa_K4231016:Design

Designed by: Shi Han   Group: iGEM22_USTC   (2022-09-30)


Y.lipolytica TEF1 promoter with a regulatory sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 797
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 797
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 797
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The composite biological part was constructed by overlap pcr.


Source

All parts were amplified from the genome of Yarrowia lipolytica strain W29.

References

1.Shabbir Hussain M, Wheeldon I, Blenner MA. A Strong Hybrid Fatty Acid Inducible Transcriptional Sensor Built From Yarrowia lipolytica Upstream Activating and Regulatory Sequences. Biotechnol J. 2017 Oct;12(10). doi: 10.1002/biot.201700248. Epub 2017 Sep 12. PMID: 28731568. 2.Wong L, Engel J, Jin E, Holdridge B, Xu P. YaliBricks, a versatile genetic toolkit for streamlined and rapid pathway engineering in Yarrowia lipolytica. Metab Eng Commun. 2017 Oct 1;5:68-77. doi: 10.1016/j.meteno.2017.09.001. Erratum in: Metab Eng Commun. 2019 Aug 13;9:e00099. PMID: 29188186; PMCID: PMC5699529.